Comparison of the prevalence of enteroviruses in blood samples of patients with and without unstable angina

Aida Gholoobi, Zahra Meshkat, Akram Baghani, Maryam Sadat Alavi, Toktam Mohamadpoor, Mastoureh Momen-Heravi, Mohsen Mouhebati, Samaneh Sepahi, Sina Rostami, Mojtaba Meshkat, Arash Gholoobi

Abstract


BACKGROUND: Although the role of enteroviruses has been proved in heart diseases, extensive information is not available on the association between enteroviruses and unstable angina. In the present study, the authors compared the prevalence of enteroviruses in patients with and without unstable angina.

METHODS: Blood samples were taken from 51 patients with unstable angina and 55 patients without unstable angina or myocardial infarction that were admitted to Imam Reza and Ghaem hospitals (Mashhad, northeast of Iran). Reverse transcription polymerase chain reaction (RT-PCR) was performed using specific primers for the detection of the enteroviruses in blood samples of study subjects.

RESULTS: Patients with and without unstable angina were similar in age with mean ± standard deviation of 62.6 ± 12.8 and 59.7 ± 12.7 years, respectively (P = 0.243) and there were no differences in gender in these two groups (P = 0.174). Prevalence of the enteroviruses in patients with unstable angina was higher only in 66-80 years age group compared to the control group (patients without unstable angina, P = 0.032). There was a higher prevalence of enterovirus RNA positivity in the blood samples of women with unstable angina (75.9%) than those without unstable angina (41.7%, P = 0.011), however, no significant difference was observed in men
(P = 0.983).

CONCLUSION: Our data showed that enteroviral RNA positivity was higher in patients with unstable angina compared to those without unstable angina. However, the differences between the two groups were not statistically significant.

 

 


Keywords


Unstable Angina; Enterovirus; Reverse Transcriptase PCR

References


Mann DL, Zipes DP, Libby P, Braunwald E, Bonow RO. Braunwald's heart disease: A textbook of cardiovascular medicine. 10th ed. Philadelphia, PA: Elsevier/Saunders; 2015.

Loscalzo J. Harrison's cardiovascular medicine. New York, NY: McGraw-Hill; 2010.

Braunwald E. Unstable angina: An etiologic approach to management. Circulation 1998; 98(21): 2219-22.

Roivainen M, Alfthan G, Jousilahti P, Kimpimaki M, Hovi T, Tuomilehto J. Enterovirus infections as a possible risk factor for myocardial infarction. Circulation 1998; 98(23): 2534-7.

Reunanen A, Roivainen M, Kleemola M, Saikku P, Leinonen M, Hovi T, et al. Enterovirus, mycoplasma and other infections as predictors for myocardial infarction. J Intern Med 2002; 252(5): 421-9.

Burch GE, Tsui CY, Harb JM. Pathologic Changes of Aorta and Coronary Arteries of Mice Infected with Coxsackie B4 Virus. Exp Biol Med 1971; 137(2): 657-61.

Simmonds J, Cubitt D, Ashworth M, Burch M. Successful heart transplantation following neonatal necrotic enterovirus myocarditis. Pediatr Cardiol 2008; 29(4): 834-7.

Muir P. Enteroviruses and heart disease. Br J Biomed Sci 1993; 50(3): 258-71.

Mohamadpoor T, Nabavinia M, Gholoobi A, Alavi M, Meshkat Z. Enteroviruses in acute myocardial infarction. Iran J Public Health 2012; 41(8): 71-4.

Kandolf R, Kirschner P, Ameis D, Canu A, Erdmann E, Schultheiss H. Enteroviral heart disease: Diagnosis by in situ hybridization. In: Schultheiss HS, Editor. New concepts in viral heart disease: Virology, immunology, and clinical management. Berlin, Germany: Springer-Verlag; 1988. p. 337-48.

Wattre P, Leroy O, Dewilde A, Thery C. Coxsackie B virus infections in cardiology. Apropos of 66 cases. Pathol Biol (Paris) 1987; 35(4): 347-52.

Zoll GJ, Melchers WJ, Kopecka H, Jambroes G, van der Poel HJ, Galama JM.. General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. J Clin Microbiol 1992; 30(1): 160-5.

Pellicano R, Mazzarello MG, Morelloni S, Ferrari M, Angelino P, Berrutti M, et al. Helicobacter pylori seropositivity in patients with unstable angina. J Cardiovasc Surg (Torino) 2003; 44(5): 605-9.

Pesonen E, Andsberg E, Ohlin H, Puolakkainen M, Rautelin H, Sarna S, et al. Dual role of infections as risk factors for coronary heart disease. Atherosclerosis 2007; 192(2): 370-5.

Pesonen E, Hallman M, Sarna S, Andsberg E, Haataja R, Meri S, et al. Mannose-binding lectin as a risk factor for acute coronary syndromes. Ann Med 2009; 41(8): 591-8.

Plotkin VI, Voronel' VL, Timoshina MA, Zaripova ZA, Murina EA, Khromov-Borisov NN. Enterovirus infection as a risk factor of acute coronary syndrome and its complications. Klin Med (Mosk) 2011; 89(2): 25-9.

Plotkin VY, Voronel VL, Timoshina MA, Zaripova ZA, Azanchevskaja SV, Murina EA, et al. Op-066

enterovirus endothelial dysfunction and myocardial infarction. Int J Cardiol 2010; 140(Suppl 1): S20.


Full Text: PDF

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.